Momwe chosokoneza ma cell chimagwirira ntchito

Chosokoneza ma cell ndi chida choyesera chomwe chimagwiritsidwa ntchito nthawi zambiri kuthyola ma cell achilengedwe ndikutulutsa zinthu zam'mimba.Mfundo yogwirira ntchito ya cell breaker imachokera pa mfundo ya kusweka kwa thupi ndi makina oscillation, ndipo cholinga cha kusweka kwa selo chimatheka popereka mphamvu zokwanira kuti ziwononge mapangidwe a maselo.

Mfundo yogwirira ntchito ya cell disruptor idzafotokozedwa mwatsatanetsatane pansipa.Zigawo zazikulu za cell disruptor zimaphatikizapo wowongolera liwiro, chipinda chophwanyidwa, mpira wophwanyidwa ndi payipi ya chitsanzo, etc. Pakati pawo, woyendetsa liwiro amagwiritsidwa ntchito poyendetsa liwiro la chipinda chophwanyidwa, chomwe ndi chidebe chosungirako. zitsanzo ndi mipira yophwanyidwa, ndipo mipira yophwanyidwa imaphwanya maselo pogundana ndi zitsanzo.Musanagwiritse ntchito chosokoneza ma cell, njira yoyenera yosokoneza iyenera kusankhidwa poyamba.Zomwe zimagwiritsidwa ntchito kawirikawiri ndi mikanda yagalasi, mikanda yachitsulo ndi mikanda ya quartz.

Mfundo zazikuluzikulu pakusankha sing'anga yophwanyira ndi chikhalidwe cha chitsanzo ndi cholinga cha kuphwanya.Mwachitsanzo, kwa maselo osalimba, mikanda yaying'ono yagalasi ingagwiritsidwe ntchito kusokoneza;kwa maselo ovuta kwambiri, mikanda yachitsulo yolimba imatha kusankhidwa.Panthawi yophwanyidwa, ikani chitsanzo kuti chiphwanyidwe mu nkhokwe yophwanyidwa, ndi kuwonjezera kuchuluka koyenera kwa sing'anga yophwanya.Kenako, liwiro lozungulira la chipinda chophwanyidwa limayendetsedwa ndi wowongolera liwiro, kotero kuti sing'anga yophwanyidwa ndi zitsanzo zikhale ndi kugunda kwamakina kosalekeza.Kugunda kumeneku kumatha kusokoneza kapangidwe ka selo kudzera mu kutengera mphamvu, kusokoneza ma cell membranes ndi organelles, ndikutulutsa zida za intracellular.

Njira yogwirira ntchito ya cell disruptor makamaka imaphatikizapo zinthu zotsatirazi: liwiro lozungulira, kukula ndi kachulukidwe ka sing'anga yophwanyidwa, kuphwanya nthawi ndi kutentha.Choyamba ndi liwiro lozungulira.Kusankhidwa kwa liwiro la kasinthasintha kuyenera kusinthidwa molingana ndi mitundu yosiyanasiyana ya ma cell ndi zitsanzo.

Kawirikawiri, kwa maselo ofewa, kuthamanga kwapamwamba kozungulira kungasankhidwe kuti awonjezere kugunda kwafupipafupi ndipo motero kusokoneza maselo bwino kwambiri.Kwa ma cell olimba, popeza ndi olimbikira, kuthamanga kwa spin kumatha kuchepetsedwa kuti muchepetse kusokonezeka kwa zitsanzo.

Chachiwiri ndi kukula ndi kachulukidwe ka sing'anga yophwanya.Kukula ndi kachulukidwe ka sing'anga yophwanyidwa kudzakhudza mwachindunji kuphwanya.Zing'onozing'ono zosokoneza zofalitsa zimatha kupereka malo ochulukirapo, zomwe zimapangitsa kuti zikhale zosavuta kusokoneza ma cellular.Kuphwanya kwakukulu media kumafuna nthawi yayitali yophwanya.

Kuonjezera apo, kachulukidwe ka sing'anga yophwanyidwa idzakhudzanso mphamvu ya kugunda, kukanika kwambiri kungayambitse kugawanika kwakukulu kwa chitsanzo.Nthawi yosokoneza ndi gawo lofunikira pakuwonongeka kwa ma cell.Kusankhidwa kwa nthawi yophwanyidwa kuyenera kutsimikiziridwa molingana ndi mtundu wa chitsanzo ndi zotsatira zophwanya.Nthawi zambiri, nthawi yayitali yosokoneza, maselo amasokonekera bwino, koma amathanso kuwononga mbali zina zachitsanzo.Chomaliza ndi kuwongolera kutentha.Zotsatira za kutentha pakugawanika kwa maselo sizinganyalanyazidwe.Kutentha kwambiri kungayambitse kusinthika kwa mapuloteni ndi ma nucleic acid m'maselo, zomwe zimakhudza kugawikana kwake.Choncho, tikulimbikitsidwa kuchita kusokonezeka kwa selo pansi pa zinthu za cryogenic, zomwe zingathe kuchepetsedwa pogwiritsa ntchito chiller kapena kugwira ntchito pa ayezi.

Zosokoneza ma cell zimagwira ntchito yofunikira pakufufuza kwachilengedwe.Mwa kuwongolera moyenera magawo monga liwiro lozungulira, kukula ndi kachulukidwe ka sing'anga, kuphwanya nthawi ndi kutentha, kuphwanya bwino kwa maselo kungathe kuchitika.Maselo akasweka, mitundu yosiyanasiyana ya zinthu m'maselo imatha kupezeka, monga mapuloteni, ma nucleic acid, ma enzyme, ndi zina zambiri, zomwe zimapereka maziko ofunikira pakuwunika ndi kafukufuku wotsatira.Mwachidule, chosokoneza ma cell ndi chida chofunikira choyesera, ndipo mfundo yake yogwirira ntchito imachokera pa mfundo ya kusweka kwa thupi ndi kugwedezeka kwamakina.Kusokoneza bwino kwa maselo kungapezeke mwa kulamulira magawo osiyanasiyana monga kuthamanga kwa kasinthasintha, kukula ndi kachulukidwe kameneka kameneka, kusokoneza nthawi ndi kutentha.Chosokoneza ma cell chimagwiritsidwa ntchito kwambiri, kupereka mwayi ndi chithandizo kwa ofufuza pa kafukufuku wokhudzana ndi biology.